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imikhiqizo

Ikhithi ye-Magnetic Bead DNA Extraction Kit Esheshayo Esebenzayo Ephumelelayo Yokuqoqa Iqoqo Lokuhlanza I-DNA

Incazelo emfushane:

I-CY-F006-10 (50preps cells)

I-CYF006-11 (100preps-cell)

I-CY-F006-12 (200preps-cell)

I-CY-F006-20 (50preps-saliva)

I-CY-F006-21 (100preps-saliva)

I-CY-F006-22 (200preps-saliva)

Ukucaciswa kokupakisha:

Abantu abangu-50/ibhokisi, abantu abayi-100/ibhokisi, abantu abangama-200/ibhokisi


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isingeniso Somkhiqizo:

I-Iclean DNA Extraction Kit inikeza indlela yobuhlalu obuzibuthe obusheshayo nephumelelayo yokuhlanza nokukhipha i-DNA (okuhlanganisa i-genomic, mitochondrial kanye ne-viral DNA) ezicutshini ezigciniwe, amathe, uketshezi lomzimba, namaseli esikhumba, omlomo wesibeletho, wesikhumba, iseli lebhaktheriya njll.

Isifanekiso sebhayoloji Amasampula angagcinwa endaweni yethu yokulondoloza ekhethekile kuze kube yizinsuku ezingu-30 ekamelweni lokushisa ngaphambi kokucutshungulwa ngaphandle kokulahlekelwa okubonakalayo ekukhiqizeni kwe-DNA noma ikhwalithi(>izinsuku ezingu-30 uma isitolo sisesimweni esibandayo)

Ikhwalithi ephezulu ye-genomic DNA ingahlanzwa emizuzwini eyi-15 ngaphandle kokukhipha i-phenol/chloroform noma imvula yotshwala, ngesivuno esimaphakathi se-DNA esingu-8 μg nge-swab ngayinye ye-buccal.I-Purified DNA, ecishe ibe ngu-20-30 kb, ilungele izinhlelo zokusebenza ezansi nomfula njenge-PCR noma okunye ukusabela kwe-enzymatic.

Izinzuzo Zomkhiqizo

Ukusebenza kahle okuphezulu, ukukhishwa okukodwa kwe-DNA, ukukhishwa okuphezulu kwamaprotheni angcolile nezinye izinhlanganisela eziphilayo kumaseli.Izingcezu ze-DNA ezikhishiwe zinkulu, zihlanzekile, zizinzile futhi zithembekile ngekhwalithi.

1. Ubuchwepheshe obusekelwe kubuhlalu obuzibuthe bokuhlukanisa i-DNA ye-genomic ngaphandle kwesidingo samakhemikhali ayingozi, i-centrifugation, noma ama-vacuum manifolds, i-phenol nemvula ye-ethanol.

2.Ukuhlanzwa okusheshayo nokuphumelelayo kwe-DNA ye-genomic kusuka kuma-buccal swabs emaminithini angaphansi kwe-15 kulandela ukulungiswa kwesampula kanye ne-lysis.

3.I-lysis elula ne-Proteinase K ngaphandle kwesidingo sanoma iyiphi i-lysis mechanical.

4.Ukungcola okuncane nge-RNA.

5.I-genomic DNA ehlanzekile ibonisa ukusebenza okuthuthukisiwe komfula ezinhlelweni ezihlanganisa i-PCR.

6.Ihlanganisa ikhithi edizayinelwe ukucutshungulwa okuzenzakalelayo kwezinombolo ezinkulu zamasampuli kumapuleti anemithombo engu-96 kusetshenziswa irobhothi elibamba uketshezi.

Ukusetshenziswa komkhiqizo:

Ukuze kuhlanzwe futhi kuhlukaniswe i-DNA (kubandakanya i-genomic, mitochondrial, bacterial, parasite & viral DNA) ezicutshini, amathe, uketshezi lomzimba, kanye ne-buccal, isibeletho, amaseli esikhumba, amangqamuzana e-bacterial, izicubu, amaswabs, i-CSF, uketshezi lomzimba, amaseli omchamo ahlanziwe. .

Imiyalo yomkhiqizo

1. Ngaphambi kokusebenzisa ama-nucleic acid extraction reagents

①.Dlulisela isincibilikisi se-proteinase k kumpushana oqandisiwe oqukethe i-proteinase k futhi uhlanganise kahle.

②.Engeza u-18ml no-42ml we-ethanol ephelele ku-CY3 kanye ne-CY4 yemodeli CY-F006-10 (50preps-cells) kanye ne-CY-F006-20 (50preps-saliva), futhi uhlanganise kahle.

③.Engeza u-36ml no-84ml we-ethanol ephelele ku-CY3 kanye ne-CY4 yemodeli CY-F006-11 (100preps-cells) kanye ne-CY-F006-21 (100preps-saliva) futhi uhlanganise kahle.

 

2. Izinyathelo zokukhishwa kweswab:

①Iqoqo elomile le-swab, engeza uketshezi lwe-CY1 elingu-0.6ml, i-10ul proteinase K, xuba kahle, ulibeke ku-incubator yomoya engu-65 degrees Celsius bese ufukamela imizuzu engu-30 (noma iqoqo elimanzi: isampula ye-centrifuge tube equkethe i-swab plus preservation solution i-centrifuged at 12000 rpm umzuzu owodwa , Gcina imvula, khipha amandla amakhulu Faka u-0.6ml CY1 liquid, 10ul proteinase k, uhlanganise kahle, ukubeke ku-incubator yomoya engu-65 degrees Celsius bese ufukamela imizuzu engu-30).

②.Susa i-swab ne-centrifuge ku-12000rpm i-1min.

③.Khipha wonke amandla amakhulu uwafake eshubhuni elisha le-centrifuge bese wenza isilingo.

④.Engeza uketshezi oluyi-0.25ml CY2, ubuhlalu obuzibuthe obuyi-10ul* (bunyakaziswa kahle ngaphambi kokusetshenziswa), hlanganisa kahle imizuzu engu-12, bubeke endaweni enozibuthe futhi buyeke bume ama-30s, bese umunca uketshezi.

⑤ Faka u-0.6ml we-CY3 liquid, uhlanganise kahle imizuzu engu-3, ​​uyibeke endaweni enozibuthe bese uyiyeka ime imizuzu engu-30 ukuze umunce uketshezi.

⑥.Faka u-0.6ml we-CY4 liquid, hlanganisa i-3min, uyibeke endaweni enozibuthe bese uyiyeka ime i-30s, ncela uketshezi.

⑦.Phinda izinyathelo ②⑥

⑧.Yomisa i-10-20min ekamelweni lokushisa, engeza i-50ul CY5 liquid for elution, uhlanganise kahle, uyibeke endaweni enozibuthe futhi uyivumele ime i-30s, bese udlulisela uketshezi eshubhuni elisha le-centrifuge.

⑨.Kala i-OD

 

3. Isinyathelo sokukhipha amathe

① I-Centrifuge ku-12000rpm i-1min namathe kanye nengxube yokulondoloza

② Gcina imvula futhi ususe amandla amakhulu

③.Faka u-0.6ml we-CY1 liquid kanye ne-10ul ye-proteinase k kuyo, uhlanganise kahle, futhi uyifake ku-air incubator engu-65 degrees Celsius bese ufukamela imizuzu engu-30.

④ I-Centrifuge ku-12000rpm i-1min, khipha konke okunamandla eshubhuni elisha le-centrifuge, engeza ubuhlalu kazibuthe obuyi-10ul kanye no-0.25ml CY2, uhlanganise kahle i-12min, uyibeke endaweni yokumisa kazibuthe futhi uyiyeke ime imizuzu engu-30 ukuze imunce uketshezi.

⑤ Faka u-0.6ml we-CY3 liquid, uhlanganise kahle imizuzu engu-3, ​​uyibeke endaweni enozibuthe bese uyiyeka ime imizuzu engu-30 ukuze umunce uketshezi.

⑥.Faka u-0.6ml we-CY4 liquid, uhlanganise i-3min, uyibeke endaweni enozibuthe bese uyiyeka ime imizuzu engu-30 ukumunca uketshezi.

⑦.Phinda isinyathelo ⑥

⑧.Yomisa ekamelweni lokushisa imizuzu engu-10-20, engeza i-50ul ye-CY5 liquid for elution, uhlanganise kahle, uyibeke endaweni yamagnetic futhi uyivumele ime i-30s, bese udlulisela uketshezi eshubhu elisha le-centrifuge.

⑨.Kala i-OD

Qaphela: Uma udinga ukususa i-RNA, ungalungisa i-RNaseA 10mg/ml: i-solvent (10mM sodium acetate: pH5.0), uyibilise imizuzu engu-15, ulungise i-pH 7.5 nge-Tris-Hcl, futhi uyigcine ku- -20 degrees Celsius.

Izimo zesitoreji nesikhathi sokuqinisekisa

1. Umkhiqizo kufanele usetshenziswe endaweni ehlanzekile nehlanzekile, egwema ukungcola, futhi enezinga lokushisa elifanele.

2. Gcina ekamelweni lokushisa.I-proteinase K kanye nobuhlalu obuzibuthe bungagcinwa ku-2-8°C isikhathi eside.

3. Impilo yeshelufu yomkhiqizo: izinyanga eziyi-12

Izinyathelo zokuzivikela:

1. Lo mkhiqizo usetshenziselwa kuphela ukuxilongwa kwe-in vitro.

2. Indawo yokugcina kanye nezinyathelo zokukhipha kufanele zilandele ngokuqinile imiyalelo ekubhukwana.

3. Uma uthola ukuthi inani lincane kakhulu ngesikhathi sokukhipha, ungakwazi ukukhulisa ngokufanelekile usayizi wesampula noma ukhuphule inani lokukhipha.

4. I-DNA ekhishiwe kufanele ibe yintsha futhi ihlolwe ngesikhathi.

Qaphela: Le ndlela yokuthutha isetshenziselwa ukuxilongwa kwe-in vitro, futhi ayikwazi ukusetshenziselwa ukusetshenziswa kwangaphakathi noma kwangaphandle kubantu noma ezilwaneni.Uma igwinywe, ingabangela izigameko ezimbi;kuyacasula emehlweni nasesikhumbeni.Uma ichaphaza ngephutha emehlweni, hlambulula ngamanzi.Kufanele kungene umoya ngesikhathi sokusetshenziswa.

Isingeniso somkhiqizi

I-Huachenyang (Shenzhen) Technology Co., Ltd. igxile ekukhiqizeni ama-swabs aminyene, ama-swabs omphimbo, ama-oral swabs, ama-nasal swabs, ama-swabs omlomo wesibeletho, ama-sponge swabs, amashubhu esampula egciwane, izixazululo zokulondoloza amagciwane.Inamandla athile embonini.okuhle

Sineminyaka engaphezu kwengu-12+ Yolwazi Lokukhiqiza Kokuthengiwe Kwezokwelapha

I-HCY ithatha ikhwalithi yomkhiqizo njengento ebalulekile ekuthuthukisweni kwebhizinisi, ukunamathela kuthishanhloko "wemikhiqizo yezinga lokuqala, izinsizakalo ezisezingeni lokuqala" ngendlela ehlanganisa yonke indawo, ephishekela umoya webhizinisi "wokufuna iqiniso, ukusungula izinto ezintsha, ubunye nokusebenza kahle" .I-HCY ihlela yonke inqubo yokukhiqiza nokudayisa ngokuhambisana ngokuqinile ne-ISO9001 kanye nohlelo lokuphatha lwe-ISO13485, ngokusebenza okuzinzile kanye nekhwalithi ethembekile.


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